TAXONOMICAL STUDIES ON SOME CULTIVATED ORANGE CULTIVARS IN EGYPT

Abstract

The present study was carried out at Horticulture and Genetic Departments Faculty of Agriculture , Moshtohor, Zagazig University on twenty orange cultivars (Yafawi,Roga­ Mozambeik,DaemEI-Haml ,Hamline ,Tuncy, Sentennial, Unani Mebattat, Sangawine ,Mazizi, W.Naval, Suckary, Mefreda, Valencia, Tanarif, Balady blood, Khalily White, Khalily Red Balady-Golden naged). Experiments were carried out during 200 I, and 2002 seosons to differentiate and separate between them through the following three categories. 1. The chromosomal behavior:

The cultivars which showed the maximum average number of univalent chromosomes were Mazizi, Mefreda, Tanarif and Golden Naged, respectively). The cultivars which showed the minimum average number of univalent chromosomes were the Tuncy, Valencia, Khalily Red and Balady, respectively). The rest of cultivars showed the median average number of univalent chromosomes per pollen mother cell. 2. SDS PAGE analysis for the studied orange cultivars:

The control marker revealed a maximum of 21 bands. Bands number I, 2, 3, 4, 5, 7, 8 and 9 were found in all cultivars. The other bands were polymorphic. It was noted that some bands are specifically linked with some cultivars. The data of SDS-PAGE of water soluble protein were applied to the computer SPSS-10 program to get a dendogram for genetic distances and similarity matrix as shown inFigure(6 ) and Table (5). The highest similarity appeared between Daem el-Hamel and Mosambiek, Tuncy and Hamline, Mefreda and Hamline and Mefreda and Tuncy as it reached 100 %.