Effect of antigenic mass and MDA on the efficacy of H5 inactivated avian influenza vaccine in commercial broiler chicken

Abstract

Propagation of reassortant low pathogenic avian influenza virus A\chicken\Egypt\Q1995D\2010 (H5N1) and A\Duck\Egypt\M2583D\2010 (H5N1) on 9-11 days old SPF-ECEs through allantoic cavity revealed highest infectivity titer of virus was 10 log10 EID50/ml and11 log10 EID50/ml, respectively. HA titer was 10 log2 HAU/ml and11 log2 HAU/ml, respectively. The harvested AIV (H5N1) fluids was inactivated by formalin at a final concentration of 0.1 % observed complete inactivation after 18hr from treatment. Assessment of Quality control measures for the prepared inactivated AIV(H5N1) vaccine indicated that, the prepared vaccines are completely sterile haven't any bacterial or fungal contaminants when tested on specific bacteriologic and fungal media. In addition, there is no local, systemic reaction or mortalities were recorded in inoculated chicks, and these denote to the safety of the prepared vaccines.