Evaluation of the diagnostic and therapeutic roles of non-coding RNA and Cell proliferation related gene association in Hepatocellular carcinoma

Abstract

A B S T R A C T miR-34a-5p is an important molecule that can inhibit the tumor growth. Bioinformatics analysis indicated minichromosome maintainance protein 2 (MCM2) was a target gene of miR-34a-p. This study aimed to investigate the functional role of miR-34a-5p in hepatocellular carcinoma (HCC) and explore the interaction between miR-34a-5p and MCM2. RT-qPCR was employed to detect the expression of miR-34a-5p and MCM2 in 10 HCC tissues. Results showed miR-34a-5p expression in HCC tissues was significantly lower than in non HCC liver tissues (P<0.05), but MCM2 expression in HCC tissues was markedly higher than in non HCC liver tissues (P<0.05). In addition, miR-34a-5p expression was negatively related to MCM2 expression. miR-34a-5p mimic and inhibitor was transfected into HCC cell lines (HepG2). CellTiter 96® AQueous One Solution Cell Proliferation Assay, showed miR-34a-5p over-expression could inhibit the proliferation of HCC cells. RT-qPCR was employed to detect the expression of miR-34a-5p and MCM2 in HepG2 cells before and after transfection. Results showed miR-34a-5p expression in HepG2 cells was significantly higher in mimic transfected group than in inhibitor transfected group and control group (P<0.05), but MCM2 expression in HCC tissues was markedly lower in mimic transfected group than in inhibitor transfected group and control group (P<0.05). Taken together, the results suggested that miR-34a-5p inhibited tumor growth by targeting MCM2.