STUDY THE EFFECT OF PURSLANE, GARDEN ROCKET AND CRESS SEEDS ON ACUTE RENAL INJURY OF RATS

Abstract

This study was conducted to acquaintance the effect of pueslane, garden rocket and cress seeds usage on biological evaluation, biochemical analysis and histopathological examination of kidneys on experimental rats suffered from acute renal injury.Forty eight mature male albino rats weighing 150±10g was used. After the adaptation period, rats were divided randomly into two main groups; the first group (negative control (C-)): 6 normal rats fed on basal diet and the second group (n= 42 rats) were injected in their hind limbs with glycerol dissolve in saline solution (50% w/v) at (10ml/kg body wt.) to induce acute renal injury. Rats were divided into seven subgroups (each group consisted of 6 rats and fed on basal diet) as following: the first subgroup was left as a positive control, the second was fed on 5% purslane seeds diet, the third was fed on10% purslane seeds, the fourth was fed on 5% garden rocket seeds diet, the fifth was fed on 10% garden rocket seeds, the sixth fed on 5% cress seeds diet and the seventh was fed on 10% cress seeds. All the above mentioned experimental groups were maintained on their corresponding diets for 28 days. At the end of experiment rats were sacrificed and collected blood samples for analysis. Biological evaluations: feed intake, body weight gain % and feed efficiency ratio and relative organs weight were recorded. Biochemical analysis: kidney function indicators, some liver enzymes, lipid profile, serum sodium and potassium levels were estimated. Histopathological change for kidney was examined. Also, the chemical composition of purslane, garden rocket, cress seeds was estimated. The results indicated to; purslane seeds (5% & 10%), garden rocket seeds (5%, 10%) and cress seeds(5% & 10%) induced appreciable homeostasis for liver and kidney functions, lipid profile, sodium and potassium levels also for kidney tissue changes. According to the results, purslane, garden rocket and cress seeds could be used for ameliorated acute renal injury.