Trials to control brucellosis using molecular biology techniques
Marwa Salah El-Din Taha Diab;
Abstract
Brucellosis is a global zoonosis of both public and economic
importance caused by the bacteria of the genus Brucella. The aim of
this study was to try some modern technologies for control of
brucellosis. In the present study Matrix-Assisted Laser Desorption
Ionization- Time Of Flight Mass Spectrometry (MALDI-TOF-MS) was
evaluated as a tool for Brucella identification that examined Brucella
reference strains and local isolates were identified by traditional
methods, multiplex PCR and MALDI-TOF-MS. Also pCI-Omp31 was
produced to evaluate its efficacy as DNA vaccine in BALB/c mice and
recombinant EryC protein was produced to compare its sero-diagnostic
efficacy with SBP and HSE as coating antigens for IELISA
importance caused by the bacteria of the genus Brucella. The aim of
this study was to try some modern technologies for control of
brucellosis. In the present study Matrix-Assisted Laser Desorption
Ionization- Time Of Flight Mass Spectrometry (MALDI-TOF-MS) was
evaluated as a tool for Brucella identification that examined Brucella
reference strains and local isolates were identified by traditional
methods, multiplex PCR and MALDI-TOF-MS. Also pCI-Omp31 was
produced to evaluate its efficacy as DNA vaccine in BALB/c mice and
recombinant EryC protein was produced to compare its sero-diagnostic
efficacy with SBP and HSE as coating antigens for IELISA
Other data
| Title | Trials to control brucellosis using molecular biology techniques | Other Titles | محاولات للسيطرة على مرض الاجهاض المعدي (البروسيلوزس) باستخدام البيولوجيا الجزيئية | Authors | Marwa Salah El-Din Taha Diab | Issue Date | 2018 |
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