BACTERIOLOGICAL ASSESSMENT OF THE INFECTED WOUNDS IN FARM ANIMALS BEFORE AND AFTER SURGICAL MANAGMENT

Abstract

This work was carried out on 87 animals of different spec1es. These animals were divided into two categories. The first category includes 72 clinical cases (50 donkeys, 4 sheep, 4 goats, 3 cows, 3 buffaloes and 8 camels). It was found that wounds of the back represented 38.88% of wounds, followed by abdomen 19.45%, head and neck 18.05%, thoracic limb 12.5% and lastly pelvic limb wounds 11.12%. The causative microorganisms responsible for wound infection were isolated and identified. It was found that the most prevalent microorganisms isolated from clinical cases were Staph. sp. (55.56%), E.coli (43.06%), Strept. pyogenes (38.89%), Corynae, pyogenes (23.81 %), Proteus vulgaris (19.44%), and Pseudomonas pyocaenous (11.11%). Antibiogram of the isolated microorganisms revealed that the Nalidexic acid was the best antibiotic of choice followed by Flumequine, Lincospectin and Neomycin. All isolates showed resistance to Ampicillin and Colistin-sulphate. The MIC of herbs was determined in vitro. It was found that the lowest concentration inhibited growth of the tested organisms of Matricaria chamomilla, Thymus vulgaris, Juniperus communis and Origanum vulgare were 10%, 10%, 15% and 5% respectively. The second experimental category included 15 clinically healthy donkeys of various ages and sexs. Infected wounds were created at various regions of the body [neck, back, gluteal and pelvic limb] in both sides of the animal. The wounds were left without treatment for 72 hours. The animals were divided into 5 groups (each of3 donkeys). The wounds of the right side were kept as control and treated only with normal saline.