Isolation, Molecular Identification and Pathogenesis of Fowl Adenovirus
Mohamed Mounir Abd El-Fattah Radwan;
Abstract
Abstract
This study was performed to detect fowl adenovirus (FAdV) circulating in commercial meat-type chicken in Egypt during 2015 and to isolate and identify the pathogenicity of the isolated virus. Cloacal swabs were collected from nine commercial broiler farms from chickens of 3-5 weeks of age in Behira province. FAdV was isolated on chicken embryo liver cells. The virus was identified by conventional polymerase chain reaction targeting a conserved region in the hexon gene. Moreover, phylogenetic analysis of the L1 loop of the hexon gene revealed that the isolated viruses clustered with reference strains belonging to FAdV serotype 8a. This is the first record of FAdV from Egypt with complete genotyping. The isolated virus is closely related to strains directly associated with inclusion body hepatitis (IBH) causing considerable economic losses. Pathogenicity study of the virus did not show any mortality, although necropsy and histopathological examination displayed severe hepatitis and degenerative changes in the primary and secondary organs of the immune system after 5 days from infection, proving that the virus can cause IBH with intermittent shedding.
This study was performed to detect fowl adenovirus (FAdV) circulating in commercial meat-type chicken in Egypt during 2015 and to isolate and identify the pathogenicity of the isolated virus. Cloacal swabs were collected from nine commercial broiler farms from chickens of 3-5 weeks of age in Behira province. FAdV was isolated on chicken embryo liver cells. The virus was identified by conventional polymerase chain reaction targeting a conserved region in the hexon gene. Moreover, phylogenetic analysis of the L1 loop of the hexon gene revealed that the isolated viruses clustered with reference strains belonging to FAdV serotype 8a. This is the first record of FAdV from Egypt with complete genotyping. The isolated virus is closely related to strains directly associated with inclusion body hepatitis (IBH) causing considerable economic losses. Pathogenicity study of the virus did not show any mortality, although necropsy and histopathological examination displayed severe hepatitis and degenerative changes in the primary and secondary organs of the immune system after 5 days from infection, proving that the virus can cause IBH with intermittent shedding.
Other data
| Title | Isolation, Molecular Identification and Pathogenesis of Fowl Adenovirus | Other Titles | عزل و توصيف جزيئي و العدوى بفيروس الأدينو في الدجاج | Authors | Mohamed Mounir Abd El-Fattah Radwan | Issue Date | 2018 |
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