Genetic comparative studies of Pasteurella infection in rabbit

Abstract

Abstract In current study, 135 samples were collected from clinically diseased rabbits of different ages from private farms of different Governorates of Egypt. The samples were collected aseptically from rabbits suffering from various signs of respiratory disorders (nasal discharges, sneezing, coughing, conjunctivitis and other respiratory disorders(. All samples were subjected to bacteriological examination, biochemical identification and molecular characterization of the isolates using Conventional PCR. 32 out of 135 samples (23.7%) were positive for P. multocida and 34 out of 135 samples (25.18%) were positive for M. haemolytica by conventional bacteriological method. PCR was confirmed that all isolates were P. multocida, and by molecular capsular typing found that all isolates were belonged to serpogroup A, the positive isolates by PCR subjected to antibiotic sensitivity testing, and the drug of choice was gentamicin. It was selected 3 strains for 16s rRNA gene sequencing and it showed genetic relation to different P . multocida subspp. with identity % = 99% . Phyloenetically, the 3 sequenced 16s rRNA gene of the Egyptian P. multocida in this study were separated into 2 different clades, the sequence with accession no. MH703779 locates in clade D that is related to the Chinese strain with accession no. EU918692. while, the 2 other sequences of the Egyptian P. multocida with accession no. MH703781 and MH703782 are related to clade C .