Anti-Angiogenic Efficacy of Chitosan Nanoparticles on Mice Bearing Ehrlich Carcinoma and Subjected to Gamma radiation

Abstract

The present study, was carried out to evaluate the anticancer activity of CNPs either alone or combined with γ-irradiation. As tumor have systemic effects on the host, studying the antioxidant status for tumor, liver, kidney and spleen, angiogenic growth factors (TNF-α, PDGF and VEGF). Also, histopathological and apoptotic examination of tumor, liver, kidney, and spleen tissues were performed to evaluate the histopathological and apoptotic changes. In vitro the cytotoxicity of nanoparticles not only led to the death of Ehrlich carcinoma cells, but also led to brust of these dead cells at certain doses. Nanoparticles led to rupture of cells content after their death. The median lethal concentration of CNPs was 20 μg/ml for Ehrlich carcinoma cells.