( GENETICAL AND BIOCHEMICAL STUDIES 1 0N DETERMINATE FABA BEAN (Viciafaba L

Abstract

The main objectives of the present investigation was to study the inheritance of some quantitative traits in faba bean as well as evaluation of some new promising inbred lines by using factorial mating design analysis (lines x testers analysis). Nine genotypes of faba bean (Vicia jaba L.) were used as parents. They were included two groups according to the growth habits, first group, four indeterminate cultivars (Giza 461, Giza 843, Sakha I and Nubaria I) were used as females and second group, five lines (D5, 06, 07, 08 and 09) were used as males. In 1999/2000 season, the parental genotypes were sown under the insect free plot in three successive planting dates to secure synchronism of flowering periods for parental genotypes. Twenty crosses were made between determinate and indeterminate genotypes in a factorial mating design without

reciprocals (female by male). In 2000/2001 season, the F

plants of twenty

successful crosses were grown and the harvested seeds furnished F2 seeds throw selfing. The same crosses repeated to produce more F1 seeds. In 200 I /2002 season. two adjacent experiments were conducted, the first involved the nine parental genotypes and their 20 F1 generation, the second involved the nine parental genotypes and their 20 F2 generati0n. Both experiments were grown in a Randomized Complete Block Design with four replications. The experimental plot consisted ofone, one and three ridges for each parent, F1 and F2 generation respectively. Each ridge was two meters long and 60 em apart and 20 em between hills on. one side of the ridge and single seed per hill. In each replication, data were recorded on 10 guarded plants in each plot for both parents, F1 generation and 30 guarded plants in F2 generation. In both experiments, data were recorded on the following traits; days to first flowering, days to maturity, plant height, first pod height, no. of branches/plant, no. of pods/plant, no. of seeds/plant, no. of seeds/pod, seed yield/plant and 100-seed weight in addition evaluation of the parent and some F1 crosses through isozyme and protein analysis. Analysis of variance was carried out in each experiment. When differences among top crosses were significant, line x tester analysis according to Kempthorne (1957) was practiced for each experiment.