Study on the production of certain 2-deoxystreptamine containing aminoglycoside antibiotics

Abstract

Aminoglycosides are natural or semisynthetic antibiotics that were introduced for routine clinical use. They have bactericidal effect on bacteria as they bind to the ribosomal decoding site hence cause misreading of mRNA. The central structure 2DOS is a key feature of the aminoglycoside antibiotics. Accordingly, the goal of our study was optimization of paromomycin production through physiological optimization, genetic mutation and molecular cloning, as an attempt for molecular optimization. The study was also concerned with the partial purification of the produced antibiotic and determination of the in vitro antibacterial effects of paromomycin either alone or in combination with other antimicrobial agents.