Antitumor Efficacy of Gallium Nanoparticles and Ellagic acid on Mammary Gland Carcinoma in Rats
Amira Mamdouh Tamamm;
Abstract
Abstract
Cancer is an important mortality contributor worldwide and breast cancer is the most common among women. Despite of the numerous breast cancer therapeutic strategies, they either have limitations or sometimes are resisted by the cancer, so new approaches are needed to tackle those restrictions. Nanotechnology offers exciting leaps forward in diagnosis and treatment of cancer, especially breast cancer. The main object of this study was to investigate the effect of the newly synthesized gallium nanoparticles coated by ellagic acid (EA-GaNPs) on the induced mammary gland carcinogenesis in female rats. The antitumor efficacy of EA-GaNPs was conducted both in vitro and in vivo. In vitro study showed that EA-GaNPs inhibited human breast cancer cell line (MCF-7) proliferation with IC50 of 2.86 µg/ml. while In vivo, the administration of EA-GaNPs to DMBA-treated rats ameliorated the hyperplastic state of mammary gland carcinogenesis induced by DMBA. Additionally, EA-GaNPs administration significantly modulated the activities of ALT and AST, as well as the levels of urea and creatinine in serum. Also EA-GaNPs administration improved the antioxidant state by increasing SOD activity and GSH content, and decreasing MDA content in the mammary tissue, besides enhancing of the apoptotic activity through elevating the levels of caspase-3 and decreasing the protein intensities of AKT & PI3K. Furthermore, a significant decrease in serum TIBC accompanied with significant increase in the level of calcium were noted. Hence, it can be concluded that EA-GaNPs could be a promising potent anticancer compound.
Cancer is an important mortality contributor worldwide and breast cancer is the most common among women. Despite of the numerous breast cancer therapeutic strategies, they either have limitations or sometimes are resisted by the cancer, so new approaches are needed to tackle those restrictions. Nanotechnology offers exciting leaps forward in diagnosis and treatment of cancer, especially breast cancer. The main object of this study was to investigate the effect of the newly synthesized gallium nanoparticles coated by ellagic acid (EA-GaNPs) on the induced mammary gland carcinogenesis in female rats. The antitumor efficacy of EA-GaNPs was conducted both in vitro and in vivo. In vitro study showed that EA-GaNPs inhibited human breast cancer cell line (MCF-7) proliferation with IC50 of 2.86 µg/ml. while In vivo, the administration of EA-GaNPs to DMBA-treated rats ameliorated the hyperplastic state of mammary gland carcinogenesis induced by DMBA. Additionally, EA-GaNPs administration significantly modulated the activities of ALT and AST, as well as the levels of urea and creatinine in serum. Also EA-GaNPs administration improved the antioxidant state by increasing SOD activity and GSH content, and decreasing MDA content in the mammary tissue, besides enhancing of the apoptotic activity through elevating the levels of caspase-3 and decreasing the protein intensities of AKT & PI3K. Furthermore, a significant decrease in serum TIBC accompanied with significant increase in the level of calcium were noted. Hence, it can be concluded that EA-GaNPs could be a promising potent anticancer compound.
Other data
| Title | Antitumor Efficacy of Gallium Nanoparticles and Ellagic acid on Mammary Gland Carcinoma in Rats | Other Titles | فاعلية جزيئات الجاليوم النانونيه و حمض الايلاجيك علي سرطان الغدد الثدييه في الجرذان | Authors | Amira Mamdouh Tamamm | Issue Date | 2018 |
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