Comparative study on different types of inactivated Pasteurella vaccine for rabbits

Abstract

Snuffle disease is one of the most important health problems in rabbits. It is caused by P. multocida. A total of 116 New-Zealand rabbits were used in evaluation of four prepared polyvalent P. multocida (serotypes A: 1, A: 3, A :12 and D: 2) vaccines. First vaccine was formalized non adjuvanted Pasteurella vaccine (FV). Second one was Lipid A adjuvanted Pasteurella vaccine (AV) in which the Lipid A was self-prepared, extracted from E. coli O: 157 and evaluated by High Performance Liquid Chromatography (HPLC). Third one was Montanide TM ISA 70 VG adjuvanted Pasteurella vaccine (MV). Fourth one was Montanide TM IMS1313 VG N PR adjuvanted Pasteurella vaccine (NV) and finally control groups. Comparisons between the prepared vaccines were done by challenge test, Lysozyme activity test, IHA test and ELISA. Statistical analysis was done. MontanideTM ISA 70 VG adjuvanted Pasteurella vaccine and Lipid A adjuvanted Pasteurella vaccines were the best, followed by Formalized non adjuvanted Pasteurella vaccine then Montanide TM IMS1313 VG N PR adjuvanted Pasteurella vaccine. In conclusion, the 1st dose vaccination may need bootstring for better results. MontanideTM ISA 70 VG and Lipid A adjuvanted Pasteurella vaccines were the best two in the prepared vaccines. Vaccination and bootstring by Montanide TM ISA 70 or Lipid A adjuvanted Pasteurella vaccines give 100 % protection. Key words: P. multocida, Lipid A, Montanide, Vaccine, one-shot, HPLC.