The Biological and Biochemical Study to Mitigate the Toxicity of Manganese in Experimental Animals
Samar Lotfy Mahmoud Mohamed;
Abstract
Abstract
This study was conducted to investigate the effect of manganese toxicity by manganese chloride (MnCl2) on the experimental animals and to evaluate the efficacy of rice bran and/or ascorbic acid in attenuating the deleterious effect induced by manganese chloride toxicity. For this purpose, fifty-four healthy rabbits weighing 1655±367.07g were divided into nine groups each of six rabbits. Group 1; rabbits fed on commercial diet and normal water served as normal control. Group 2; rabbits fed on commercial diet and received 200 mg/L of MnCl2 in drinking water. Group 3; rabbits received MnCl2 in drinking water (200 mg/L) and fed on commercial diet supplemented with 200 g dried powder of rice bran added to 1 kg diet as a source for phytic acid. Group 4; rabbits fed on commercial diet and received 200 mg/L MnCl2 in drinking water and orally administered with ascorbic acid (30 mg/kg body weight daily). Group 5; rabbits received MnCl2 in drinking water (200 mg/L) and supplemented with rice bran (200 g /kg diet) and orally administered ascorbic acid (30 mg/kg body weight daily) by intragastric tube. Group 6; Rabbits were fed commercial diet and injected intravitreally with MnCl2 (25µl twice/week). Group 7; Rabbits were injected intravitreally with MnCl2 and fed rice bran (200 g /kg diet). Group 8; Rabbits were injected intravitreally with MnCl2 and were orally administered ascorbic acid (30 mg/kg body weight daily) by intragastric tube. Group 9; Rabbits were injected intravitreally with MnCl2 and fed rice bran (200 g /kg diet) and were orally administered ascorbic acid (30 mg/kg body weight daily) by intragastric tube. Results showed that MnCl2 intoxication significantly reduced haemoglobin (Hb) concentration and serum iron with a significant increase in total iron binding capacity. Also, it induced a significant increase in malondialdehyde (MDA) level accompanied by a significant decrease in reduced glutathione (GSH) concentration and superoxide dismutase (SOD) activity. Moreover, MnCl2 intoxication caused a significant increase in serum alanine transaminase (ALT), aspartate transaminase (AST) activities.
This study was conducted to investigate the effect of manganese toxicity by manganese chloride (MnCl2) on the experimental animals and to evaluate the efficacy of rice bran and/or ascorbic acid in attenuating the deleterious effect induced by manganese chloride toxicity. For this purpose, fifty-four healthy rabbits weighing 1655±367.07g were divided into nine groups each of six rabbits. Group 1; rabbits fed on commercial diet and normal water served as normal control. Group 2; rabbits fed on commercial diet and received 200 mg/L of MnCl2 in drinking water. Group 3; rabbits received MnCl2 in drinking water (200 mg/L) and fed on commercial diet supplemented with 200 g dried powder of rice bran added to 1 kg diet as a source for phytic acid. Group 4; rabbits fed on commercial diet and received 200 mg/L MnCl2 in drinking water and orally administered with ascorbic acid (30 mg/kg body weight daily). Group 5; rabbits received MnCl2 in drinking water (200 mg/L) and supplemented with rice bran (200 g /kg diet) and orally administered ascorbic acid (30 mg/kg body weight daily) by intragastric tube. Group 6; Rabbits were fed commercial diet and injected intravitreally with MnCl2 (25µl twice/week). Group 7; Rabbits were injected intravitreally with MnCl2 and fed rice bran (200 g /kg diet). Group 8; Rabbits were injected intravitreally with MnCl2 and were orally administered ascorbic acid (30 mg/kg body weight daily) by intragastric tube. Group 9; Rabbits were injected intravitreally with MnCl2 and fed rice bran (200 g /kg diet) and were orally administered ascorbic acid (30 mg/kg body weight daily) by intragastric tube. Results showed that MnCl2 intoxication significantly reduced haemoglobin (Hb) concentration and serum iron with a significant increase in total iron binding capacity. Also, it induced a significant increase in malondialdehyde (MDA) level accompanied by a significant decrease in reduced glutathione (GSH) concentration and superoxide dismutase (SOD) activity. Moreover, MnCl2 intoxication caused a significant increase in serum alanine transaminase (ALT), aspartate transaminase (AST) activities.
Other data
| Title | The Biological and Biochemical Study to Mitigate the Toxicity of Manganese in Experimental Animals | Other Titles | دراسة بيولوجية وكيميائية للحد من سمية المنجنيز في حيوانات التجارب | Authors | Samar Lotfy Mahmoud Mohamed | Issue Date | 2019 |
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