Biochemical Studies on Chitosan as Cisplatin Carrier in Hepatocellular Carcinoma in Rats

Abstract

Natural polymers of polysaccharide type like chitosan, which was used as a carrier for the several chemotherapies such as cisplatin which is more stable, bio adhesive, bioactive and more permeable. The chitosan was extracted from the shrimp shells by using the chemical reagents. Anew carrier used for delivering the cisplatin to the carcinoma part of the liver tissue which is dual ligand binded with the chitosan as a carrier. The dual vector is composed from chitosan, lactobionic acid and glycyrrhetinic acid in the presence of (EDC·HCl) and NHS for activating the carboxylic groups of glycyrrhetinic acid and lactobionic acid through 2 main steps under normal conditions. In previous study, the results documented the potential effect of dual ligand for delivery the drug to the cancer part of the liver. In this study the designed drug was been the cisplatin-alginate/dual ligand nanoparticles which loaded with strongest chemotherapy, cisplatin for treating hepatocellular carcinoma in rats. The results were been indicated that the nanoparticles have higher antitumor efficacy in the liver cancer treatment and lower side effect compared to free CDDP. In conclusion, this designed drug was suppressing liver tumor tissue by releasing rapidly a high concentration of cisplatin inside the liver cancer cells, thus increasing the efficacy of the drug