CHEMISTRY AND BIOLOGICAL STUDY OF LEAVES EXTRACT OF PISTACIA ATLANTICA DESF. GROWING IN LIBYA

Abstract

In accordance with the recent worldwide interest in plant phenolics, which emerges from their broad range of biological activities, particular emphasis has been focused, in the present thesis, on the constitutive phenolics of the extract of pistacia atlantica desf. (Ancardiaceae) , Libya. The present study was divided into two parts: Part I: Phytochemical investigation,included isolation , identification of the phenolic constituents of the ethyl acetate leaf extract of p. atlantica which has not been subjected previously to a comprehensive phytochemical study. Part II: Biological investigation of pistacia atlantica leaf extract In vitro investigation including, antioxidant, antibacteria , and cytotoxic activities of the ethyl acetate leaf extract of pistacia atlantica desf. and some of the isolated pure compounds as well. Part I: Phytochemical investigation of p.atlantica desf., leaf extract: The phytochemical study of p.atlantica desf., included successive column chromatographic investigation of the ethyl acetate leaf extract, separation of individual phenolics, repeated purification of these individuals and establishment of their homogeneity by chromatographic analysis. For the structure elucidation of the isolated phenolics, the required structural information was obtained through chromatographic analysis, application of chemical degradation methods and conventional spectroscopic techniques of analysis as well.