Biocontamination in poultry vaccines

Abstract

Abstract Freedom of poultry vaccines from biocontamination (bacterial and viral contaminants) should be secured throughout the process of production and before market release to avoid infection of immunized poultry. The principle cause of contamination of poultry vaccines is using embryonated chicken eggs as a substrate for propagation of vaccine virus. The detection of vaccine contaminates by the classical methods is expensive, time consuming and in most cases lack sensitivity, so in the present study we used new tests that are simple, sensitive, specific, rapid and give results in short possible time as lateral flow immunochromatographic test (LFIT) and polymerase chain reaction (PCR). For detection of Salmonella Enteritidis, the minimal bacterial count that gave positive result of LFIT and PCR was 10 CFU/0.1 ml and 10 CFU/ 0.1 ml respectively. For detection of some viral contaminates, it was found that PCR test can differentiate between hemagglutinating viruses and identify between the subgroups of avian leukosis virus.