NOVEL CHROMATOGRAPHIC METHODS FOR ASSESSMENT OF SOME HEPATIC DRUGS AND APPLICATIONS TO PHARMACOKINETIC AND STATISTICAL STUDIES

Abstract

In summary, a rapid, specific, reproducible, high-throughput and sensitive UPLC-MS/MS method allows determination of Adefovir using Adefovir-d4 as an internal standard was developed and validated. Overall the developed method presented adequate sensitivity, excellent selectivity and desired reproducibility for the quantification of Adefovir in human plasma. The additional significant advantage of this validated method is the shorter runtime obtained (1.5 min), that allow the analysis of over 500 samples per day. Moreover, the low limit of quantitation (1 ng/ml), the low matrix effect, the precision and accuracy of the method and also the stability are well within the limits required for bioanalytical assays. In addition, the ISR result with 96.64% at the end of the study further added strength to our current method.