PHYSIOLOGICAL AND MOLECULAR STUDIES ASSOCIATED WITH ENGINNERING DEHYDRATION- STRESS TOLERANCE IN SESAME
AMAL FAROUK ABD ELHAMIED ALSHAFEAY;
Abstract
Drought is a major stress that globally restricting the agricultural expansion
and crops productivity. Sesame (Sesamum indicum L.) considered as a
recalcitrant plant to be regenerated. In this study for improving the dehydration
stress tolerance of sesame plant, a LOS5 of Arabidopsis thaliana was introduced
to de-embryonated cotyledons of sesame cv. Sohag1 via Agrobacterium
tumefaciens strain LBA4404 which harboring the LOS5 gene encode a
molybdenum cofactor sulfurase (MCSU), which catalyzes the generation of the
sulfurylated form of MoCo, a cofactor required by aldehyde oxidase to
functions in the last step of ABA biosynthesis, leading to ABA accumulation in
tissues followed by enhancing the drought stress tolerance. The insertion of
AtLOS5 was confirmed by PCR and RT-PCR analysis. When sesame plants
were exposed to various level of water stress (50% and 25% of pot irrigation
capacity), transgenic sesame plantlets showed slower water loss and cell
membrane damage due to the reduced content of malondialdehyde (MDA)
compared to non- transgenic plants. Transgenic sesame accumulated 2 fold
more proline than azygous plants. Transgenic sesame also exhibited a
significant increase in the antioxidant enzymes activity such as catalase,
superoxide dismutase, peroxidase, and ascorbate peroxidase. When LOS5
transgenic sesame subjected to sever water stress (25% of irrigation), transgenic
plants wilted partially however the non- transgenic plants almost died. The yield
and its components of transgenic lines were higher than azygous plants. Under
both 50 and 25% water stress, Under either water stress level, transgenic lines A
and B showed higher significant values of seed or capsule yield/ plant as well as
number of capsule/ plant as comparing to that obtained from transgenic line C
or azygous plants. All the above mentioned observation could be a consequence
of the improving of sesame drought tolerance through enhancing the ABA
production which induced cascade of stress-up-regulated genes and
osmoprotectant like proline and other antioxidant enzyme which responsible of
many physiological and biochemical regulations.
and crops productivity. Sesame (Sesamum indicum L.) considered as a
recalcitrant plant to be regenerated. In this study for improving the dehydration
stress tolerance of sesame plant, a LOS5 of Arabidopsis thaliana was introduced
to de-embryonated cotyledons of sesame cv. Sohag1 via Agrobacterium
tumefaciens strain LBA4404 which harboring the LOS5 gene encode a
molybdenum cofactor sulfurase (MCSU), which catalyzes the generation of the
sulfurylated form of MoCo, a cofactor required by aldehyde oxidase to
functions in the last step of ABA biosynthesis, leading to ABA accumulation in
tissues followed by enhancing the drought stress tolerance. The insertion of
AtLOS5 was confirmed by PCR and RT-PCR analysis. When sesame plants
were exposed to various level of water stress (50% and 25% of pot irrigation
capacity), transgenic sesame plantlets showed slower water loss and cell
membrane damage due to the reduced content of malondialdehyde (MDA)
compared to non- transgenic plants. Transgenic sesame accumulated 2 fold
more proline than azygous plants. Transgenic sesame also exhibited a
significant increase in the antioxidant enzymes activity such as catalase,
superoxide dismutase, peroxidase, and ascorbate peroxidase. When LOS5
transgenic sesame subjected to sever water stress (25% of irrigation), transgenic
plants wilted partially however the non- transgenic plants almost died. The yield
and its components of transgenic lines were higher than azygous plants. Under
both 50 and 25% water stress, Under either water stress level, transgenic lines A
and B showed higher significant values of seed or capsule yield/ plant as well as
number of capsule/ plant as comparing to that obtained from transgenic line C
or azygous plants. All the above mentioned observation could be a consequence
of the improving of sesame drought tolerance through enhancing the ABA
production which induced cascade of stress-up-regulated genes and
osmoprotectant like proline and other antioxidant enzyme which responsible of
many physiological and biochemical regulations.
Other data
| Title | PHYSIOLOGICAL AND MOLECULAR STUDIES ASSOCIATED WITH ENGINNERING DEHYDRATION- STRESS TOLERANCE IN SESAME | Other Titles | دراسات فسيولوجيه و جزيئيه على نباتات السمسم المهندسه وراثيًا لمقاومه الإجهاد بالجفاف | Authors | AMAL FAROUK ABD ELHAMIED ALSHAFEAY | Issue Date | 2019 |
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