PHYSIOLOGICAL STUDIES ON JOJOBA PLANT [Simmondsia chinensis (Link) Schneider] USING TISSUE CULTURE TECHNIQUES
BASSANT MUBARAK MOHAMED HAMDY;
Abstract
The current work was conducted to improve the regeneration capacity
of jojoba plants from callus derived from female leaf explants. Therefore, an
improved culture medium was developed and used in the main experiments
of regeneration via induction of adventitious shoot buds. Thus, leaf explants
of female jojoba plants were cultured on an improved media for regeneration
of jojoba plants; callus induction medium (CIM), bud formation medium
(BFM), shoot development medium (SDM) and root development medium
(RDM), containing various concentrations of plant growth regulators, auxin
(2,4-D, Dicamba, NAA or IBA) either alone or in combination with cytokinin
(BA or TDZ). The obtained results revealed that, the use of the combination
between Dicamba (1.5 mg/l) and TDZ (0.75 mg/l) in CIM produced the
highest percentage of callus induction (98.65 %). In addition, the use of TDZ
(2.5 mg/l) in combination with Dicamba (0.5 mg/l) in BFM produced the
highest percentage of shoot regeneration (99.65%) with the highest average
number of the produced shoots/callus (23.18). The results of root formation
elucidated that, the inclusion of BA (1 mg/l) in combination with (IBA 1.5
mg/l + NAA 1.5 mg/l) led to a significant increase in the percentage of shoots
produced roots and recorded the highest level (100.00 %) and the highest
average number of regenerated roots/plantlet (7.11) compared to the
combination between (IBA 1.5 mg/l + NAA 1.5 mg/l) which recorded (58.13
%) and (4.38 roots/plantlet). Accordingly, the average number of regenerated
roots/plantlet was positively correlated with the percentage of the survival
rate of the acclimatized jojoba plants, whereas the highest survival rate (96.41
%) was recorded for the plants with the highest average number of
regenerated roots/plantlet (7.11). These results proved that, a highly efficient
regeneration protocol of female jojoba plants via adventitious shoot bud
formation from callus derived from female leaf explants was achieved.
Furthermore, this improved protocol of jojoba regeneration is of great
importance for establishing a reliable protocol for Agrobacterium
tumefaciens-mediated transformation of jojoba plants.
of jojoba plants from callus derived from female leaf explants. Therefore, an
improved culture medium was developed and used in the main experiments
of regeneration via induction of adventitious shoot buds. Thus, leaf explants
of female jojoba plants were cultured on an improved media for regeneration
of jojoba plants; callus induction medium (CIM), bud formation medium
(BFM), shoot development medium (SDM) and root development medium
(RDM), containing various concentrations of plant growth regulators, auxin
(2,4-D, Dicamba, NAA or IBA) either alone or in combination with cytokinin
(BA or TDZ). The obtained results revealed that, the use of the combination
between Dicamba (1.5 mg/l) and TDZ (0.75 mg/l) in CIM produced the
highest percentage of callus induction (98.65 %). In addition, the use of TDZ
(2.5 mg/l) in combination with Dicamba (0.5 mg/l) in BFM produced the
highest percentage of shoot regeneration (99.65%) with the highest average
number of the produced shoots/callus (23.18). The results of root formation
elucidated that, the inclusion of BA (1 mg/l) in combination with (IBA 1.5
mg/l + NAA 1.5 mg/l) led to a significant increase in the percentage of shoots
produced roots and recorded the highest level (100.00 %) and the highest
average number of regenerated roots/plantlet (7.11) compared to the
combination between (IBA 1.5 mg/l + NAA 1.5 mg/l) which recorded (58.13
%) and (4.38 roots/plantlet). Accordingly, the average number of regenerated
roots/plantlet was positively correlated with the percentage of the survival
rate of the acclimatized jojoba plants, whereas the highest survival rate (96.41
%) was recorded for the plants with the highest average number of
regenerated roots/plantlet (7.11). These results proved that, a highly efficient
regeneration protocol of female jojoba plants via adventitious shoot bud
formation from callus derived from female leaf explants was achieved.
Furthermore, this improved protocol of jojoba regeneration is of great
importance for establishing a reliable protocol for Agrobacterium
tumefaciens-mediated transformation of jojoba plants.
Other data
| Title | PHYSIOLOGICAL STUDIES ON JOJOBA PLANT [Simmondsia chinensis (Link) Schneider] USING TISSUE CULTURE TECHNIQUES | Other Titles | دراسات فسيولوجية على نبات الجوجوبا باستخدام تقنيات زراعة الأنسجة | Authors | BASSANT MUBARAK MOHAMED HAMDY | Issue Date | 2018 |
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