Simultaneous Detection of MRSA, Selected gram negative bacilli, clinically relevant Candida species and Aspergillus by PCR in Critically ill Patients with Sepsis

Abstract

P ediatric severe sepsis remains a burdensome public health problem. Sepsis was the most expensive condition treated in U.S. hospital stays in 2011. We aimed to use PCR for rapid detection of the organisms in critically ill patients with sepsis for rapid diagnosis and start the appropriate treatment and compare its results with the conventional blood culture results. This study was a cross-sectional observational study that carried out in Pediatric ICU, Children’s Hospital, Ain Shams University. This study included 45 critically ill patients in pediatric age group (30days-18years) with sepsis or septic shock. Patients were subjected to revising of records, PIM2 score and routine laboratory results. Microbiological analysis: Bacterial culture and antibacterial sensitivity test, fungal culture and antifungal sensitivity test. PCR: blood samples were obtained and DNA was extracted from the samples using Qiagen DNA tissue kit for MRSA, Acinetobacter, Pseudomonas, E coli, Klebsiella, Candida species and Aspergillus.