DETERMINATION OF SOME ENVIRONMENTAL TOXINS THAT MIGHT CAUSE LIVER CANCER IN FOOD
Hend Mohamed Ali Ahmed;
Abstract
Abstract
Human are exposed to hepatocarcinogenic aflatoxins through ingestion of foods which exposed to poor storage condition of susceptible grains or eating foods carried aflatoxins from animals and vegetables. This study evaluated the effect of total aflatoxin on liver health of subjects. Ninety Subjects were divided into three groups (each /30 Subjects). Group (1) not suffering liver disease as normal or negative control. Group (2) patients input/or output (El Raghi hospital in Assuit) are suffering of hepatocellular carcinoma (HCC). Group (3) patients input (El Raghi hospital in Assuit) are suffering of liver inflammatory disease (HI). This study used 24 h recall and food frequency questioners for these groups to determination the type and amount of food which were intake. Random samples were obtained of some foods frequency intake as a source of protein (chicken egg; chicken, duck and rabbit liver) purchased from local market in Assuit; which determined total amount of aflatoxins using high- performance liquid chromatography (HPLC). In serum was measured Aflatoxin Albumin Adduct (AAA) by enzyme-linked immunosorbent assay (ELISA) kites and used colorimetric kites to determine liver function (Alanine transaminase (ALT), Aspartate transaminase (AST), Total protein (TP), albumin (Alb), Bilirubin (Total, Direct), alkaline phosphatase (ALP), Glutathione peroxidase (GPX) and Malondialdehyde (MDA) by used spectroscopy instrument. Study detected a correlation between ALT, GPx, MDA, and AAA in human.
Study concluded that these alterations in liver functions could be related to the development of liver damage in response to significant dose of Aflatoxins.
Human are exposed to hepatocarcinogenic aflatoxins through ingestion of foods which exposed to poor storage condition of susceptible grains or eating foods carried aflatoxins from animals and vegetables. This study evaluated the effect of total aflatoxin on liver health of subjects. Ninety Subjects were divided into three groups (each /30 Subjects). Group (1) not suffering liver disease as normal or negative control. Group (2) patients input/or output (El Raghi hospital in Assuit) are suffering of hepatocellular carcinoma (HCC). Group (3) patients input (El Raghi hospital in Assuit) are suffering of liver inflammatory disease (HI). This study used 24 h recall and food frequency questioners for these groups to determination the type and amount of food which were intake. Random samples were obtained of some foods frequency intake as a source of protein (chicken egg; chicken, duck and rabbit liver) purchased from local market in Assuit; which determined total amount of aflatoxins using high- performance liquid chromatography (HPLC). In serum was measured Aflatoxin Albumin Adduct (AAA) by enzyme-linked immunosorbent assay (ELISA) kites and used colorimetric kites to determine liver function (Alanine transaminase (ALT), Aspartate transaminase (AST), Total protein (TP), albumin (Alb), Bilirubin (Total, Direct), alkaline phosphatase (ALP), Glutathione peroxidase (GPX) and Malondialdehyde (MDA) by used spectroscopy instrument. Study detected a correlation between ALT, GPx, MDA, and AAA in human.
Study concluded that these alterations in liver functions could be related to the development of liver damage in response to significant dose of Aflatoxins.
Other data
| Title | DETERMINATION OF SOME ENVIRONMENTAL TOXINS THAT MIGHT CAUSE LIVER CANCER IN FOOD | Authors | Hend Mohamed Ali Ahmed | Issue Date | 2018 |
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