Potential Modulatory Effect of Eugenol on Insulin Resistance in Experimental Model of Fatty Liver in Rats

Abstract

This study was assembled to explore the potential protective effect of EUG on IR in NAFLD induced by HFCD diet, and to clarify the potential underlying mechanisms of EUG via studying its effect on different oxidative stress, inflammatory, fibrotic markers. This was achieved by establishing a rat model of NASH with fibrosis by using a HFCD diet. Rats were randomly divided into four groups; Group (I) served as control group, fed normal rat chow diet and received olive oil (10mg/kg) orally 3 times weekly for 8 weeks . Group (II) considered as (HFCD) group fed HFCD diet and received olive oil (10 mg/kg) orally 3 times weekly for 8 weeks. Group (III) served as (HFCD+EUG) group fed HFCD diet and received (10 mg/kg) of EUG orally 3 times weekly for 8 weeks. Group (IV) considered as (EUG) only group fed normal rat chow diet and received (10 mg/kg) EUG orally 3 times weekly for 8 weeks.1 At the end of the 8th week, blood samples were collected for measuring blood glucose level and also for biochemical assessment. Then, rats of all groups were sacrificed, where liver tissues were dissected out and divided into two parts; parts were used for histopathological examination, while other parts were dissected out for further analysis for assessment of inflammatory, oxidative stress, and fibrotic markers.