Regenerative Potential of Cultured Gingival fibroblast- Mesenchymal Stem Cells in Treatment of Periodontal Intrabony Defects (Randomized Clinical and Biochemical Trial)

Abstract

eriodontitis is an infectious disease of the periodontal tissue that can lead to tooth loss in adults. The lost periodontium, including both hard (alveolar bone and cementum) and soft (periodontal ligament) tissues, needs to be restored to its original architecture. The complex microenvironment of the periodontal wound creates many challenges associated with the regeneration of multiple tissue types within periodontal lesions. Guided tissue regeneration (GTR) with barrier membranes has been established to be effective in periodontal regeneration. GTR has several important fuctions including; blood clot stabilization, ability to attain primary intention wound healing, keep the defect isolated from the gingival fibroblasts and their extracellular matrix (ECM), and space maintenance. During a GTR procedure, the membrane excludes any contribution of mesenchymal stem cells and osteoblasts from the periosteum and gingival tissue. The human gingival tissue contains numerous types of cells, like gingival fibroblasts and gingival mesenchymal cells. Fibroblasts are abundant mesenchymal cells that play important roles in development and tissue repair. Recent studies