Immunohistochemical Characterization for Perforation Repair Material

Abstract

The aim of the present study was to compare between MTA, Biodentine, and Experimental tricalcium silicate bone cement regarding bone loss, inflammatory tissue reaction and new hard tissue formation after repair of the furcal perforation defect. This study included radiographic, histological, and immunohistochemical analysis; for evaluating the treatment outcomes over 3month evaluation period. A total of 162 teeth from 12 healthy adult dogs were selected. All teeth were intact, and had complete root development. The dogs were divided into three main experimental groups of 54 teeth, 4 dogs each, according to the evaluation period. Each group was further divided into two equal Subgroups of 27 teeth, 2dogs each, according to the time of repair. Each of these two Subgroups was further subdivided into five subdivisions according to the material used; MTA, Biodentine, Experimental material, positive control and negative control. After anesthetizing the dogs, the teeth were cleaned, shaped and obturated. Perforations were created in the center of the maximum curve of the pulp chamber floors. The perforations in Subgroup (A) were sealed immediately while; the perforations were left open for 1month for salivary contamination in Subgroup (B). No perforations were made in the negative control group (Subdivision 5) while the perforations were left open without repair in the positive control group (Subdivision 4). The three study materials used were; MTA (Subdivision 1), Biodentine (Subdivision 2), and Experimental tricalcium silicate cement (Subdivision 3). Then the animals were returned to the animal house in; Surgery, Anesthesiology and Radiology department, Cairo University, and were left for follow up according to the post treatment evaluation period; 1month (GI), 2month (GII), and 3month (GIII). At the end of each evaluation period, the dogs were reanaesthesized and postoperative radiographs were taken.