Curcumin-Simvastatin Release Profile in the Gingival Crevicular Fluid Following EDTA Root Surface Etching in the Surgical Treatment of Intrabony Periodontal Defects – A Biochemical and Clinical Study

Abstract

eriodontal disease is a common oral disease that is identified by severe inflammation and loss of the periodontal attachment apparatus. Regenerative periodontal therapy aims to restore the tooth’s supporting periodontal tissues that have been lost due to periodontal disease. The objective of the present study is to evaluate the effect of EDTA root surface etching on simvastatin and curcumin availability in the GCF and to evaluate the influence of EDTA-curcumin-simvastatin on the clinical and radiographic outcome of the surgical treatment of intrabony periodontal defect. Demineralization of root surface with 24% EDTA removes smear layer and exposes collagen fibers. Simvastatin exerts anabolic effects on bone beside its anti-resorptive actions. Moreover, it has anti-inflammatory and antimicrobial properties. Curcumin enhances wound healing and it has anti-inflammatory, antioxidant, and antimicrobial effects. The present study entailed thirty patients having stage II or stage III, Grade A periodontitis with 30 intrabony defects. Patients were divided into three groups; group I was subjected to open flap debridement only. Group II was subjected to open flap debridement followed by application of curcumin-simvastatin paste (2%curcumin and 1.2 %simvastatin). While