IN VIVO (MICE) EFFECTS OF SOME METABOLIC INHIBITOR (S) ON THE GROWTH RATE OF INDUCED CARCINOMA

Abstract

The present study was undertaken to determine the antiturnour activity of the metabolic inhibitor iodoacetate (IAA) and/or dimethylsulphoxide (DMSO) on solid Ehrlich carcinoma in vivo. Toxicity study was carried out to determine the LDso value of JAA and was found to be (7.35 mg/1 00 g B.W.). Treatment of tumour-bearing mice with LD 125 values of IAA (1.84 mg/100 g B.W.) and/or DMSO (0.35 g/100 g B.W.) every other day elongated the life span, increased significantly the survivors' % and the cumulative mean survival times as well as reduced the mean tumour weights of tumoured-treated animals. In addition, the tumour growth inhibition ratio (T/C %) and tumour growth delay time (T-C value) were also increased. The most notable tumour inhibitory effect was seen in the combined treatment. Results obtained from biochemical studies revealed that lAA treatment per se significantly increased the levels of plasma lactate dehydrogenase (LDH) activity and liver glycogen, while significantly decreased the levels of liver total proteins, RNA and DNA, compared to normal controls. On the other hand, DMSO treatment. significantly elevated the activity of blood antioxidant enzymes, such as erythrocytes glutathione peroxidase (GPx) and glucose-6-phosphate dehydrogenase (G6PDH), :while blood catalase activity was insignificantly changed. In contrast DMSO decreased liver RNA and DNA concentrations. Combined treatment of turnoured animals significantly increased the levels of plasma LDH and erythrocytes G6PDH activities and liver glycogen and in contrast decreased the levels of liver total proteins, RNA and DNA, compared to normal controls.