PROTECTIVE EFFECTS OF CACTUS AND/OR PAPAYA JUICES AGAINST HEPATIC AND TESTICULAR TOXICITY INDUCED BY CHLORPYRIFOS IN ALBINO RATS

Abstract

This study aimed to evaluate the effect of cactus and/orpapaya juiceson chlorpyrifos induced toxicity inratʼs liver and testes.A total of sixty albino rats weighing (180g -200g) were used for this study. Rats were divided into 5groups each of 12rats. Firstgroup wasleft as control group.Second group was given chlorpyrifos 6mg/kg bwt.The other three groups were given cactus, papaya juices and both of them as 20ml/kg bwt, subsequently along with chlorpyrifos dose.All doses were orally administered daily for 28successive days. At the end of the experimental period, blood samples, liver and testes were collected from each rat for biochemical analysis. Oral administration of chlorpyrifos for 28days in a dose of 6mg/kg bwt, significantlyincreasedthe percentage of DNA fragmentation, caspase-3 level inliver and testes tissues. Asignificant increase in serum alanine aminotransferase,aspartate aminotransferase, catalase, cytochrome P450, paraxonase-1 activity as well astumor necrosis factor-α, malondialdehydeand follicle stimulating hormoneslevelsand decrease in testosterone and luteinizing hormonelevels was observed. On the other hand the administration of tested chlorpyrifoswith juices, reduce the percentage of DNA fragmentation, caspase-3 level and directed enzymes activity and hormones levels toward normal. The effect on the liver is one of the main toxic effects of this product. In conclusion cactus and papaya juices havebeneficial effects as it tends to dampen chlorpyrifos toxicity in rats.