lncRNA- RP11-156p1.3, novel diagnostic and therapeutic targeting via CRISPR/Cas9 editing in hepatocellular carcinoma
Ali, Hebatalla Said; Boshra, Mariam Sameh; Mahmoud El-Meteini; Shafei, Ayman; Matboli, Marwa;
Abstract
We aim to characterize the expression of RNA panel in HCC. We assessed the expression of HCC-associated mRNA, miRNA and lncRNA network by real time PCR in sera and tissue samples. In a proof-of-principle approach, CRISPR cas9 mediated knock out for lncRNA- RP11-156p1.3 was performed in HEPG2 cell line to validate the role of the chosen RNA in HCC pathogenesis. The differential expression of RFTN1 mRNA, lncRNA- RP11-156p1.3 and miRNA-4764-5p was statistically different among the studied groups. After CRISPR cas9 mediated knockout of lncRNA- RP11-156p1.3 in HEPG2 cells, there was significant decrease in cell count and viability with reversal of the expression of the chosen RNAs. The chosen RNAs play a significant role in HCC pathogenesis and may be potential diagnostic and therapeutic targets.
Other data
| Title | lncRNA- RP11-156p1.3, novel diagnostic and therapeutic targeting via CRISPR/Cas9 editing in hepatocellular carcinoma | Authors | Ali, Hebatalla Said; Boshra, Mariam Sameh; Mahmoud El-Meteini ; Shafei, Ayman ; Matboli, Marwa | Affiliations | Faculty of medicine | Keywords | CRISPR cas9,Diagnosis;Hepatocellular carcinoma;Therapy;lncRNA;miRNA | Issue Date | Sep-2020 | Publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | Journal | Genomics | Volume | 112 | Issue | 5 | Start page | 3306 | End page | 3314 | ISSN | 0888-7543 | DOI | 10.1016/j.ygeno.2020.06.020 | PubMed ID | 32544548 | Scopus ID | 2-s2.0-85086518910 | Web of science ID | WOS:000547954200020 |
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