Developing a biomaterial interface based on poly(lactic acid) via plasma-assisted covalent anchorage of d-glucosamine and its potential for tissue regeneration
Swilem, Ahmed; Lehocký, Marian; Humpolíček, Petr; Kucekova, Zdenka; Junkar, Ita; Mozetič, Miran; Hamed, Ashraf A; Novák, Igor;
Abstract
The aim of this study was to develop the potential tissue engineering applications of d-glucosamine (GlcN) immobilized onto the surface of a biodegradable matrix in order to induce a desired biological effect at biointerfaces. Thus, for sample preparation we used a novel multistep physicochemical approach. In the first step the poly(lactic acid) (PLA) films were exposed to a low pressure plasma in air atmosphere, followed by radical graft copolymerization with acrylic acid to yield a carboxyl-functionalized spacer layer on the PLA surface. The carboxyl groups were then coupled to GlcN molecules via the carbodiimide chemistry. The developed surfaces were characterized by X-ray Photoelectron Spectroscopy (XPS), Contact angle measurements and Atomic Force Microscopy (AFM). A preliminary study on the proliferation of fibroblasts on the developed surfaces was performed using the NIH/3T3 cell line.
Other data
Title | Developing a biomaterial interface based on poly(lactic acid) via plasma-assisted covalent anchorage of d-glucosamine and its potential for tissue regeneration | Authors | Swilem, Ahmed ; Lehocký, Marian; Humpolíček, Petr; Kucekova, Zdenka; Junkar, Ita; Mozetič, Miran; Hamed, Ashraf A; Novák, Igor | Keywords | Carbodiimide;Fibroblast viability;Plasma treatment;Poly(lactic acid);d-Glucosamine | Issue Date | 1-Dec-2016 | Journal | Colloids and surfaces. B, Biointerfaces | Volume | 148 | Start page | 59 | End page | 65 | ISSN | 09277765 | DOI | 10.1016/j.colsurfb.2016.08.046 | PubMed ID | 27591571 |
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