Resistance to Colistin Mediated by mcr-1 among Multidrug Resistant Gram Negative Pathogens at a Tertiary Care Hospital, Egypt
Shabban, Marwa; Alaa Eldin Fahim, Noha; Montasser, Karim; El Magd, Nagwa M Abo;
Abstract
Colistin is considered the last option for treatment of infections caused by Multidrug Resistant (MDR) Gram
negative pathogens. The mcr-1 gene could transfer the resistance to colistin between different species.
Therefore, screening of this resistance mechanism will help greatly in the control of further spread of colistin
resistance and enhancing the outcomes of patients. The current work aimed to study the frequency of
colistin resistance among MDR Gram negative pathogens isolated from clinical samples at Ain Shams
University Hospitals and to explore if the mcr-1 gene was the responsible mechanism for this resistance.
These pathogens were isolated from various samples including; blood, pleural fluid, sputum, urine,
swabs from surgical and burn wounds, that were submitted from wards and intensive care units to the
central microbiology laboratory at Ain Shams University Hospital, Cairo, Egypt, during the period from
June to December 2019. Culture and bacterial identification were done by conventional microbiologic
techniques. Eighty Gram negative bacterial pathogens were assayed for antimicrobial susceptibility by
disc diffusion test. Sixty MDR Gram negative isolates were identified and further studied for colistin
susceptibility by E- test, as well as real- time PCR to detect mcr-1 gene. Totally four isolates (6.7%)
were phenotypically resistant to colistin. We found mcr-1 gene in three of the obtained isolates (5%);
1 Pseudomonas aeruginosa (5.3%) and 2 Acinetobacter baumannii (14.3%). In conclusion, although
we detected a low prevalence of mcr-1 positive isolates from human clinical samples, continuous
monitoring and implementation of infection control precautions are greatly required, to interfere with
the further occurrence and transfer of bacterial species carrying the mcr-1 gene.
negative pathogens. The mcr-1 gene could transfer the resistance to colistin between different species.
Therefore, screening of this resistance mechanism will help greatly in the control of further spread of colistin
resistance and enhancing the outcomes of patients. The current work aimed to study the frequency of
colistin resistance among MDR Gram negative pathogens isolated from clinical samples at Ain Shams
University Hospitals and to explore if the mcr-1 gene was the responsible mechanism for this resistance.
These pathogens were isolated from various samples including; blood, pleural fluid, sputum, urine,
swabs from surgical and burn wounds, that were submitted from wards and intensive care units to the
central microbiology laboratory at Ain Shams University Hospital, Cairo, Egypt, during the period from
June to December 2019. Culture and bacterial identification were done by conventional microbiologic
techniques. Eighty Gram negative bacterial pathogens were assayed for antimicrobial susceptibility by
disc diffusion test. Sixty MDR Gram negative isolates were identified and further studied for colistin
susceptibility by E- test, as well as real- time PCR to detect mcr-1 gene. Totally four isolates (6.7%)
were phenotypically resistant to colistin. We found mcr-1 gene in three of the obtained isolates (5%);
1 Pseudomonas aeruginosa (5.3%) and 2 Acinetobacter baumannii (14.3%). In conclusion, although
we detected a low prevalence of mcr-1 positive isolates from human clinical samples, continuous
monitoring and implementation of infection control precautions are greatly required, to interfere with
the further occurrence and transfer of bacterial species carrying the mcr-1 gene.
Other data
Title | Resistance to Colistin Mediated by mcr-1 among Multidrug Resistant Gram Negative Pathogens at a Tertiary Care Hospital, Egypt | Authors | Shabban, Marwa; Alaa Eldin Fahim, Noha ; Montasser, Karim; El Magd, Nagwa M Abo | Keywords | Colistin resistance;Real- time PCR;Multidrug resistant Gram negative pathogens;mcr-1 gene | Issue Date | Jun-2020 | Publisher | Dr. M.N. Khan | Journal | Journal of Pure and Applied Microbiology | Volume | 14 | Issue | 2 | Start page | 1125 | End page | 1132 | ISSN | 09737510 2581690X |
DOI | 10.22207/JPAM.14.2.07 |
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