Modulatory effect of rutin on Deca-Durabolin- induced reproductive damage via suppression of oxidative stress and apoptosis in adult male rats.

Abstract

Deca- Durabolin (Nandrolone decanoate, ND) treatment causes damage in the male reproductive system. Rutin (RT) is a naturally occurring flavonoid glycoside that has antioxidant and anti-inflammatory properties. This study aimed to investigate effects of rutin against ND-induced reproductive toxicity in male rats. Forty adult male rats were used. The control group received olive oil with oral gavage during 6 weeks. ND Groups received different doses (25, 50, 100 mg Kg-1 , i.m.). RT + ND groups received RT (50 mg kg -1 , P.o.) during 6 weeks, associated with (25,50,100 mg Kg-1 , i.m.). Biochemical (total protein, testosterone, MDA, SOD and CAT), histological, histochemical and immunohistochemical studies of testicles were evaluated. ND treatment increased the oxidative stress and induced testicular degeneration and apoptosis when compared to the control group. However, RT treatment relieves these side effects when compared to the ND group alone. It is concluded that RT treatment may reduce ND-induced reproductive toxicity as apotential antioxidant compound.