Simultaneous spectrophotometric determination of drugs lacking peak maxima in their zero-order profiles by graphical or statistical representation of data
Ragaa Magdy; A. Hemdan; Nermine Victor Fares; Maha Farouk;
Abstract
Trandolapril has no sharp peak in its zero-order spectrum, therefore it is difficult to be
measured by direct spectrophotometry. In this study, direct univariate spectrophotometric
methods were developed and validated for determination of Trandolapril and Verapamil
combination in pure and tablet dosage forms. The first method for measuring both
Trandolapril and Verapamil is Absorbance Subtraction (AS), this method depends on the
presence of iso-absorptive point in the zero-order curve at 217 nm. It has the advantage of
measuring the concentration of both Trandolapril and Verapamil from unified regression
equation at the iso-absorptive point. The second, third and fourth methods were applied on
the first order spectra of the studied drugs. Second method is Derivative Subtraction (DS) for
Trandolapril and Derivative subtraction followed by spectrum subtraction (DS-SS) for
Verapamil. The third and fourth methods are constant value and concentration value
methods. In the concentration value method, the concentration of the drugs is determined
from the graphical representation without the use of regression equations. All the developed
methods were validated as per International Conference on Harmonization guidelines and
the results proved that the developed methods are simple, accurate, and selective. Moreover,
a statistical comparison between the developed methods and a reference method was done.
Also, One-way ANOVA statistical test was done between all the proposed spectrophoto-
metric methods and results showed no significant differences.
measured by direct spectrophotometry. In this study, direct univariate spectrophotometric
methods were developed and validated for determination of Trandolapril and Verapamil
combination in pure and tablet dosage forms. The first method for measuring both
Trandolapril and Verapamil is Absorbance Subtraction (AS), this method depends on the
presence of iso-absorptive point in the zero-order curve at 217 nm. It has the advantage of
measuring the concentration of both Trandolapril and Verapamil from unified regression
equation at the iso-absorptive point. The second, third and fourth methods were applied on
the first order spectra of the studied drugs. Second method is Derivative Subtraction (DS) for
Trandolapril and Derivative subtraction followed by spectrum subtraction (DS-SS) for
Verapamil. The third and fourth methods are constant value and concentration value
methods. In the concentration value method, the concentration of the drugs is determined
from the graphical representation without the use of regression equations. All the developed
methods were validated as per International Conference on Harmonization guidelines and
the results proved that the developed methods are simple, accurate, and selective. Moreover,
a statistical comparison between the developed methods and a reference method was done.
Also, One-way ANOVA statistical test was done between all the proposed spectrophoto-
metric methods and results showed no significant differences.
Other data
Title | Simultaneous spectrophotometric determination of drugs lacking peak maxima in their zero-order profiles by graphical or statistical representation of data | Authors | Ragaa Magdy; A. Hemdan; Nermine Victor Fares ; Maha Farouk | Issue Date | 30-Sep-2018 | Publisher | Atlanta Publishing House LLC | Journal | European Journal of Chemistry | Volume | 9 | Issue | 3 | Start page | 194 | End page | 201 | DOI | 10.5155/eurjchem.9.3.194-201.1727 |
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