Optimization of Extraction, HPLC and Kinetic Studies for Determination of Some Food Tainting Compounds in Different Food Matrices

Abstract

Solid–liquid extraction, ultrasonic-assisted extraction and matrix solid-phase dispersion (MSPD) were optimized and compared in terms of recoveries for the simultaneous extraction of indole (IND) and 2,4-dichlorophenol (DCP) from catfish samples and for the extrac- tion of IND alone from potato samples. Applying high-performance liquid chromatography (HPLC-DAD) procedure using mobile phase of methanol : water (65 : 35) at 280 nm, MSPD was the method of choice for the extraction of IND and DCP from catfish and, also, for IND from potato. The extraction recoveries of MSPD were in the range (97.9–99.7%) and (99.8–100.6%); for IND and DCP, respective- ly, in catfish samples and (98.4–99.7%) for IND alone in potato sam- ples. Solid-phase extraction (SPE) was chosen the method of choice for the extraction of DCP from fish farms water samples after optimi- zation and comparison with direct sample injection and extraction recoveries were in the range (97.9–100.3%). Kinetics were further studied to follow each of production of IND in catfish during storage at different temperatures and uptake of DCP by tilapia in fish farms water samples using MSPD-HPLC and SPE-HPLC, respectively.