Determination of amlodipine and atorvastatin mixture by different spectrophotometric methods with or without regression equations
Ragaa Magdy; A. Hemdan; Nermine Victor Fares; Maha Farouk;
Abstract
Four new, simple, and reproducible spectrophotometric methods were developed and validated for the simulta-
neous determination of Amlodipine (AML) and Atorvastatin (AT) in bulk powder and pharmaceutical dosage
form. The four methods include two progressive and two successive resolution techniques. The two progressive
methods are Absorbance Subtraction (AS) and Amplitude Modulation (AM), while the two successive methods
are Constant Value (CV) and Concentration Value. In the Concentration Value method, the concentration of the
drugs is determined from the graphical representation without the use of regression equations. Linearity range
for the two progressive methods was from 5 μg/mL–35 μg/mL while for the two successive methods was from
5 μg/mL–55 μg/mL. The four methods were validated according to the ICH guidelines and were found to be accu-
rate, precise, and selective. The methods were also applied for determination of the mixture in the marketed
pharmaceutical dosage form. Results obtained were compared with reported methods. Also, One-way ANOVA
statistical test was done between all the proposed spectrophotometric methods where no significant differences
were found.
neous determination of Amlodipine (AML) and Atorvastatin (AT) in bulk powder and pharmaceutical dosage
form. The four methods include two progressive and two successive resolution techniques. The two progressive
methods are Absorbance Subtraction (AS) and Amplitude Modulation (AM), while the two successive methods
are Constant Value (CV) and Concentration Value. In the Concentration Value method, the concentration of the
drugs is determined from the graphical representation without the use of regression equations. Linearity range
for the two progressive methods was from 5 μg/mL–35 μg/mL while for the two successive methods was from
5 μg/mL–55 μg/mL. The four methods were validated according to the ICH guidelines and were found to be accu-
rate, precise, and selective. The methods were also applied for determination of the mixture in the marketed
pharmaceutical dosage form. Results obtained were compared with reported methods. Also, One-way ANOVA
statistical test was done between all the proposed spectrophotometric methods where no significant differences
were found.
Other data
Title | Determination of amlodipine and atorvastatin mixture by different spectrophotometric methods with or without regression equations | Authors | Ragaa Magdy; A. Hemdan; Nermine Victor Fares ; Maha Farouk | Issue Date | 9-Nov-2018 | Publisher | Elsevier | Journal | Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Issue | 210 | Start page | 203 | End page | 211 | DOI | 10.1016/ j.saa.2018.11.014 |
Recommend this item
Similar Items from Core Recommender Database
Items in Ain Shams Scholar are protected by copyright, with all rights reserved, unless otherwise indicated.