MODULATOR IMPACTS OF PROPOLIS EXTRACT AGAINST DOXORUBICIN MEDIATED CARCINOGENESIS ON HEPATOCELLULAR CARCINOMA AND Drosophila SOMATIC CELLS
Ali, Sawsan; Naglaa M. Ebee;
Abstract
The antitumor action of propolis is
of clinical interest because of the need for
new anticancer treatment agents. The present
investigation intended to extract and
assess the chemical content, cytotoxic
action, the growth inhibitory activity and
anticancer capability of Egyptian propolis
versus Chinese propolis. This was carried
out using water extract (WE) and
ethanolic extract (EE) on the human hepatocellular
carcinoma (HEp-2) cell line and
the loss of heterozygosity (LOH) assay of
Drosophila melanogaster somatic cells
against the direct genotoxicity of doxorubicin.
EPWE, EPEE, CPWE and CPEE
extracts analyzed by HPLC showed that
there were sensible and various concentrations
of phenolic compounds in both.
Total phenolics were determined to
be 18.83, 34.87, 39.29 and 180.89 g-1 by
using EPWE, CPWE, EPEE and CPEE
extracts, respectively. Chinese propolis
ethanol extract (CPEE) have major concentrations
of total phenolics and phenolic
acids and contained high concentrations ofrutin (188.90 g/mL). The study of the
antiproliferative capacity of propolis extractors
against HEp-2 cancer cell lines
showed that all the studied propolis extracts
induce suppression of cell growth
except CPWE extract; it gave 100% cell
viability. The great majority of the
propolis are strongly cytotoxic against
HEp-2 cell line with 500 μg/ml CPEE.
Also, PEE is the most effective in inhibition
of HEp-2 cell proliferation compared
to PWE. In Drosophila assay, treatment
with propolis extract and DOX carcinogenic
agent led to a reduction in the frequency
of recombination compared to the
treatment with DOX alone either in the
post- and pre-treatments. In general, PEE
exhibited powerful anti-proliferative effects
than PWE. The ethanol extract provided
the highest protection against Doxorubicin
(DOR) induced genotoxicity, a
fact that supports their anti-cancer activity.
The results demonstrate that PEE is a
good source of a natural antitumor operator
able to inhibit cancer cell proliferation.
of clinical interest because of the need for
new anticancer treatment agents. The present
investigation intended to extract and
assess the chemical content, cytotoxic
action, the growth inhibitory activity and
anticancer capability of Egyptian propolis
versus Chinese propolis. This was carried
out using water extract (WE) and
ethanolic extract (EE) on the human hepatocellular
carcinoma (HEp-2) cell line and
the loss of heterozygosity (LOH) assay of
Drosophila melanogaster somatic cells
against the direct genotoxicity of doxorubicin.
EPWE, EPEE, CPWE and CPEE
extracts analyzed by HPLC showed that
there were sensible and various concentrations
of phenolic compounds in both.
Total phenolics were determined to
be 18.83, 34.87, 39.29 and 180.89 g-1 by
using EPWE, CPWE, EPEE and CPEE
extracts, respectively. Chinese propolis
ethanol extract (CPEE) have major concentrations
of total phenolics and phenolic
acids and contained high concentrations ofrutin (188.90 g/mL). The study of the
antiproliferative capacity of propolis extractors
against HEp-2 cancer cell lines
showed that all the studied propolis extracts
induce suppression of cell growth
except CPWE extract; it gave 100% cell
viability. The great majority of the
propolis are strongly cytotoxic against
HEp-2 cell line with 500 μg/ml CPEE.
Also, PEE is the most effective in inhibition
of HEp-2 cell proliferation compared
to PWE. In Drosophila assay, treatment
with propolis extract and DOX carcinogenic
agent led to a reduction in the frequency
of recombination compared to the
treatment with DOX alone either in the
post- and pre-treatments. In general, PEE
exhibited powerful anti-proliferative effects
than PWE. The ethanol extract provided
the highest protection against Doxorubicin
(DOR) induced genotoxicity, a
fact that supports their anti-cancer activity.
The results demonstrate that PEE is a
good source of a natural antitumor operator
able to inhibit cancer cell proliferation.
Other data
| Title | MODULATOR IMPACTS OF PROPOLIS EXTRACT AGAINST DOXORUBICIN MEDIATED CARCINOGENESIS ON HEPATOCELLULAR CARCINOMA AND Drosophila SOMATIC CELLS | Authors | Ali, Sawsan ; Naglaa M. Ebee | Issue Date | 2017 | Journal | Egypt. J. Genet. Cytol. | Volume | 46 | Start page | 389 | End page | 407 | Description | MODULATOR IMPACTS OF PROPOLIS EXTRACT AGAINST DOXORUBICIN MEDIATED CARCINOGENESIS ON HEPATOCELLULAR CARCINOMA AND Drosophila SOMATIC CELLS |
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