DETECTION OF MICROORGANISMS (BACTERIA, FUNGI AND YEASTS) IN ROYAL JELLY

Abstract

The aim of the present study to detect the pop-ulation and frequency (%) of microorganism (bac-teria, fungi and yeasts) in royal jelly samples. The data indicated that, there are no significant differ-ences were remarked in the population of microor-ganisms between all the samples for bacteria, fun-gi and yeasts, where the mean number of popula-tion was 5.923, 1.38 and 7.85 colonies/sample for bacteria, fungi and yeasts respectively, in pro-duced royal jelly from honeybee colonies, local royal jelly collected from Egyptian market and samples of imported royal jelly collected from Egyptian market, respectively. According to the isolation and identification procedures for detected royal jelly samples, four bacteria types (Clostridium botulinum, Bacillus cereus, Bacillus wakoensis and Micrococcus luteus), two fungi types (Aspergil-lusniger and Penicillium sp.) and one yeast type (Saccharomyces cerevisiae) were determined. The data also summarized that Clostridium botulinum was the most frequency compared with the other bacterial types, where the percentage of frequency was 1.8 – 2.5, 0.9 – 1.4, 0.4 – 0.6 and 1.5 – 2.0% for C. botulinum, B. cereus, B. wakoensis and Mi-crococcus luteus, respectively. Meanwhile, Penicil-lium sp. the most frequency compared with A. ni-ger, where the percentage of frequency was 0.5 - 2.9 and 0.7 – 1.0 %, respectively, in produced roy-al jelly from honeybee colonies, local royal jelly samples collected from Egyptian market and im-ported royal jelly samples collected from Egyptian market respectively. Regarding the yeasts, the data also summarized that, S. cerevisiae was the most frequency in royal jelly that produced from honeybee colonies (2.9%) followed by which local royal jelly samples collected from Egyptian market (2.1%) and imported royal jelly samples collected from Egyptian market (1.8%).