Silver nanoparticles as antimicrobial agent from Kluyveromyces marxianus and Candida utilis

Abstract

A green approach for synthesizing the nanoparticles using the yeast have been suggested as promising ecofriendly alternative to chemical methods. The present study involves the extracellular biosynthesis of silver nanoparticles (Ag-NPs) using Kluyveromyces marxianus, Candida utilis 22 and evaluating the antibacterial and antifungal efficacy against Staphylococcuc aureus ATCC 6538, Escherichia coli ATCC 10536, Pseudomonas flourescence ATCC 50090, Candida albicans, Candida glabrata, Candida krusei as multi-drug resistant human pathogens. Morphological observation and characterization of biosynthesized silver nanoparticles were performed by UV-visible spectroscopy, Transmission electron microscopy and Fourier transform infrared spectroscopy. The biosynthesized silver nanoparticles from each yeast strains showed a maximum absorption in the visible region at 430-450 nm and at 400-430 nm respectively and the size was ranged from 3-12 nm and 6-20 nm respectively. The interaction between protein and Ag-NPs was analyzed and the stabilization of Ag-NPs by protein is a clear possibility. Further more, Ag-NPs have the highest antibacterial and antifungal efficacy against all the tested microorganisms. Conclusion, silver nanoparticles from each strains have great potential to be an effective to antibacterial and/or antifungal agents for future therapies in multi-drug resistant human pathogens of bacteria and Candida infections.