GENETICAL AND PHYSIOLOGICAL STUDIES ON JAPANESE QUAIL "Studies on the Magnitude Of Resistance of Transgenic Quails to Heat Stress"

Abstract

The present study was conducted as an attempt to introduce functional genes of adrenal gland and thyroid glands from Naked neck chickens or bacteria (Streptococcus agalactia) through its fragmented purified DNA into the embryonic cells of Japanese quail (Coturnic coturnix japonica). The effect of these treatments on the ability of hatched chicks to tolerate heat stress conditions was investigated. Hematological and biochemical parameters were evaluated. Moreover,• pathological and cytological studies were also carried out. Two thousand eight hundred and eighty eggs of Japanese quail (Coturnix coturnix japonica) (in three hatches) were used in this study. All eggs were •incubated at 37.8°C, in forced air incubator. At the 3'd day of incubation, the eggs were divided randomly into 12 experimental groups. Each group of eggs was randomly assigned to one of DNA injection treatments as follows: