Analytical study the interaction between the inhibitors of serotonin-noradrenaline receptors and some pesticides in rats

Abstract

The certified reference of Tramadol was of >99 % purity. 2) Abamectin A pure reference standard of abamectin (>98.7%, purity. Experimental Designs Nighty six (96) male albino rats (Rattus norvegicus) of the same stock were obtained from the animal house of Cairo University, Giza-Egypt. Exposure protocol At the start of the study, the male rats were approximately 10 weeks old (weight 180-200 g). The rats were randomly assigned to 3 groups of 32 animals each and were treated as follows: Group 1: Tramadol-treated rats. The animal group (Tramadol group, N1 = 32), was divided into 8 sub-groups received tramadol .Each sub-group consisted of 4 rats (n1=4). Were received Tramadol at doses of 40 mg kg-1, administered orally by gavage. Group 2: Abamectin-treated rats. The animal group (Abamectin group, N2 = 32), was divided into 8 sub-groups received abamectin .Each sub-group consisted of 4 rats (n2=4). Were received Abamectin at doses of 10 mg kg-1, administered orally by gavage. Group 3: Tramadol-Abamectin - treated rats. The animal group (Tramadol +Abamectin treated group, N3 = 32), was divided into 8 sub-groups ,each sub-group consisted of 4 rats (n3=4) and received Tramadol at a dose of 40 mg kg-1 plus Abamectin at doses of 10 mg kg-1, administered orally by gavage. Tramadol and Abamectin were administrated orally by gavages using corn oil. Samples and parameters measured 1) Blood samples Blood and plasma samples were collected at constant intervals; half hr, hr1 hr, 1.5 hr, 2 hr ,2.5 hr,3 hr 3.5hr and 24 hr, respectively. samples were used for the determination of:  Urea and creatinine  (AST) and alanine aminotransferase (ALT) enzyme activities. Brain specimens were taken at constant intervals; half hr, hr1 hr, 1.5 hr, 2 hr ,2.5 hr,3 hr 3.5hr and 24 hr respectively, for the determination the following (Biochemical analysis):