Studies on the Molecular Mechanisms of Acinetobacter Resistant Isolates to Aminoglycosides.

Abstract

Acinetobacter baumannii is an opportunistic organism that poses largely nosocomial outbreaks throughout the world. Aminoglycosides was the treatment option for Acinetobacter infections but their resistance has increased in the recent years. Regarding the emergence of multidrug resistant A. baumannii in hospitals on genetic bases of aminoglycosides modifying enzyme to give a good strategy to control multidrug resistant A. baumannii infection through the following:

Firstly: Collection, Isolation and Identification of A. baumannii Two-hundred and fifty (250) bacterial isolates were collected from different five hospitals (Nasser Institute, Al-Kasr Al-Aini Hospital, Abu El-Reesh, Al-haram Hospital, and Al Hussein Hospital) in Cairo, Egypt from different infection sites as endotracheal tube, blood, sputum, wound and urine, this ratio from endotracheal tube 47.2%, 20.4% from blood samples, 12.8% from sputum samples, 3.6% from urine and 6% from wound sample respectively and 167 bacterial isolates were isolated from female while 83 strains from male along the period of one year through November 2016 to December 2017. Out of 250 isolates two-hundred and twenty (220) 88% bacterial isolates were identified as Acinetobacter spp. based on phenotypic technique, and two-hundred 200 (90.9%) from 220 of