Study of the Possible Mechanisms for the Role of Mesenchymal Stem Cells in Treating Acute Kidney Injury in a Rat Model

Abstract

The current study was undertaken to address the therapeutic effects of mesenchymal stem cells (BM-MSCs and AD-MSCs) on AKI induced experimentally by cisplatin and tracing the mechanisms by which they act. Also, the study was extended to optimize the dosage of MSCs infusion and to explore the advantages of MSCs therapy over the conventional therapy (Losartan) in the treatment of AKI. For MSCs isolation and propagation; BM-MSCs were isolated from bone marrow of both femur and tibia of male Wistar rats. While, AD-MSCs were obtained from abdominal fats of male Wistar rats. Then, the isolated cells were grown and propagated in culture. To confirm that the cells in the culture were MSCs, they were characterized morphologically by inverted microscope examination, and by detection of their specific cell surface markers (CD29, CD166, CD45, and CD34) gene expressions which was done by conventional PCR technique.