APPLICATION OF NANOTECHNOLOGY IN NATURAL COMPOUNDS FOR ANTIOXIDANT AND ANTICANCER PREPARATIONS

Abstract

As multi-targeted compound, Thymoquinone (TQ) has been showed numerous therapeutic potentials in different diseases including cancers. Piperine has a wide range of pharmacological properties. It is used as an antioxidant, anti-cancer, etc, still not yet reach clinical use. This because some inherit limitations, as low bioavailability and solubility, targeting delivery, and selectivity. Here, in present studies have successfully tailored novel nanoformulations for TQ delivery relay on mesoporous silica nanoparticles (MSNs) as nanocarrier, and for Pip delivery relay on hydroxyapatite (HAP) as nanocarrier and made modification by adding phosphonate group (HAP-H) .TQ loaded into mesoporous of MSNs as core (MSNTQ), subsequently step, coating of the core with mixture of two different polymers-based designs to obtain shell layer (MSNTQ-WA, Whey protein-Arabic gum, and MSNTQ-CS, Chitosan-Stearic acid). The anticancer efficiency and selectivity in brain cancer cells (SW1088 and A172) compared to brain normal ones (HCN2) were investigated. The anticancer efficiency and selectivity of (HAP-Pip, HAP-H-Pip and Pip) in breast cancer cells (MCF7), colon cancer cells (CaCo2) and normal lungs (WI38) cell were used as normal cells. Furthermore . The results demonstrated that anticancer activity in term of cell viability was varied and found to be cell line- With respect of biocompatibility of nanocarrier, and less toxic on normal than cancer cells Going further, the selective index calculations based on IC50 values verified that MSNTQ-WA, HAP-H-Pip exhibited better selectivity compared to all. Collectively, as the molecular mechanisms of action are concerned, the important findings is that nanoformulations especially MSNTQ-WA allowed remarkable effect to improve caspase-3 activation, Cytochrom c triggers, cell cycle arrest at G2/M phase and HAP-H-Pip has apoptosis induction compared to Pi in brain cancer cells.