MODERN TECHNIQUES AND NOVEL METHODS IN MICROANALYSIS

Abstract

The studies presented in this dissertation were limited to the development of some new sensors based on potentiometric and amperometric transduction modes. The developed sensors were applied successfully, mainly, in phannaceutical and biomedical analyses.

In the potentiometric part, two potentiometric PVC membrane sensors for selective detennination of iodide and ferroin were developed. The iodide membrane sensor was incorporated in a flow cell and the flow system was optimized for sequential flow-injection detennination of iodide and iodine in phannaceutical preparations containing povidone iodine. The ferroin sensor \vas used for iron quantification after its simple conversion into ferroin. The sensor was applied to iron detennination in water, rocks, cement, glass, alloys, and phannaceutical preparations.

In the amperometric pmt, three different miniaturized amperometric biosensors were fabricated on flexible Kapton substrate. The first sensor was of the first generation type and was based on immobilized lactate oxidase. This sensor was used for direct continuous monitoring of extracellular lactate accumulation in ischemic rabbit myocardium. The second sensor was of the third .generation type and based on umnobilized lactate oxidase and tetrathiafulvalene-