Phenotypic and genotypic characterization of Klebsiella species recovered from camels

Abstract

Klebsiella species considered one of the most bacterial diseases affect camels, it causes respiratory manifestation, urinary tract infections and digestive problems which impair production and health. The bacteriological examination of internal organs of 150 camel from slaughterhouses of El qualiobia and El-monofia revealed isolation of 44 Klebsiella strains with an overall incidence 29.3%. The Klebsiella strains can be isolated from internal organs lung, liver, kidney and spleen of apparently healthy camel with an incidence of 10.6%,7.3%,7.3% and 4% respectively. Biochemical identification revealed that 12 isolates were K.pneumoniae (5.2%), 26 K.oxytoca (11.4%) and 6 K.rhinoscleromatis (2.6%). The in vitro sensitivity of 44 Klebsiella strains were studied to investigate resistance to 18 antimicrobial drugs. the most susceptible one was were Meropenem 95.4% followed by imipenen 81.8% and cefatizidime 54.5% but they were resistant to Clindamycin 97.7%, Trimethoprim\ sulphamethoxazole 84,8% and oxytetracycline 75%. Congo red binding assay showing different degree in red colour, 28 (63.6%) gave deep red colour and 16 strains (36.3%) gave pale orange. The mannose resistant haemagglutination pattern of K. pneumoniae isolates against erythrocytes of cattle, sheep ,horse and human (group O) were 85.3%, 66.6%,41.6%,50% respectively, while they were 50%. 33.3%, 66.6%, 66.6%for K.rhinoscleromatis and 69.2%, 57.6%, 69.2, 53.8% K.oxytoca .