Analytical Study of some drugs containing tertiary amine group

Abstract

RRLC and HPLC methods were developed for the determination butamirate citrate (BC) and itopride hydrochloride (IT) in bulk powder and pharmaceutical formulations as well as in presence of (BC) degradation product. Rapid resolution liquid chromatography (RRLC) Agilent SB-C18 (4.6×50 mm, 1.8 µm) column with mobile phase [0.2% triethyl amine bufer:acetonitrile (50:50 v/v)], pH 4.8 adjusted with phosphoric acid and ultraviolet detection at 222 nm was the first method for the determination of (BC). HPLC Promosil C18 (4.6 mm×250 mm, 5 μm) column with mobile phase [0.05 M ammonium dihydrogen phosphate bufer:acetonitrile 50:50 (v/v)] with pH 5.4 and ultraviolet detection at 222 nm was the second method. RRLC method was applied for the determination of Itopride HCl (IT) and Pantoprazole Na (PA) as mixture. Rapid resolution liquid chromatography (RRLC) Bonus- RP C18, (1.8 µm, 4.6x50 mm) column; mobile phase:(0.02% amm. formate buffer: methanol (50:50 v/v), pH 5.6 adjusted with formic acid and ultraviolet detection at 280 nm was the first method.