SOME BIOCHEMICAL AND IMMUNOLOGICAL STUDIES IN RABBITS INJECTED WITH RADIATED AND NON RADIATED ANTIGEN

Abstract

The ionizing radiation could be used in many biological researches which should be established in the area of application, as the using of the ionizing radiation in the preparation of many vaccines against many infectious diseases such as production of active irradiated vaccine against P-multocida and comparing its efficacy with the ordinary dead formalized vaccine.

The present work was clone on 72 of male New Zealand white rabbits at two months of age. They had neither a history off. multocidc infection nor vaccination. They were classified into three groups; each group contain 24 (twenty four) rabbits arranged as follow: Group .1: non vaccinated rabbits which used as a control.

Group ll: rabbits vaccinated with f. multocidc inactivated by gamma radiation with a dose of lml per rabbit. Group Ill: rabbits vaccinated with• multocide inactivated by formalin with a dose of lml per rabbit. The rabbits were subjected to revacination by a subsequent same doses at fivteen clays post initial vaccination, wherease blood and liver samples (after sacrificing of 4 rabbits) were collected from each group at 15, 25, 35, 45, clays for the assessment of serological, biochemical and immunological investigations.

The rabbits were subjected to homologous virulent strain of P. multocida 5-A (subsequent challenge test) at the 45 day after initial vaccination, the serological studies were carried out with the use of indirect 1-Jaemagglutination test (Il-IA).