Study of Antimicrobial activities of some Egyptian plants Hyphaene thebaica and Physalis peruviana

Abstract

The use of natural antimicrobial products extracted from plants is nowadays widely used, to decrease the complications rise from the use of synthetic antibiotics, so this study interested to show the antimicrobial activity of Physalis peruvianae and Hyphaene thebaica fruit extract by using four solvent (water, boiling water, ethanol 70% and acetone). The different extracts were tested to inhibitory effect by using well diffusion technique at concentrations (10, 30 and 50%) on eight bacterial strains (Escherichia coli, Proteus mirabilis, Salmonella typhimurium, Salmonella entrica , Shigella dysenteriae , Bacillus cereus, Bacillus licheniformis and Staphylococcus aureus). The study demonstrated that ethanol extract of Physalis peruvianae and water extract of Hyphaene thebaica were the best extracts, and Staph. aureus was the most sensitive gram-positive strain to both extracts with inhibition zone 23 and 24mm respectively, and E. coli was the most sensitive gram-negative strain to both extracts with inhibition zone 27 and 20mm respectively. The inhibition zone diameters of both extracts were near to the synthetic antibiotic (Ciprofloxacin). The results showed that the scavenging effects of both extracts on DPPH radicals increased by increasing the concentration and IC50 was 1322 and 771.4 µg ̸ ml respectively. Fruit extracts acquired their therapeutic activities (antimicrobial and antioxidant activities) because of their polyphenolic content such as phenolic acids (chlorogenic acid, caffeic acid, galic acid and coumaric acid) , flavoinodes (rutin, querctin , catechin and hesperidine) and high values of tannins .