Process Development of Nano-aluminum Hydroxide Adjuvanted Rift Valley Fever virus vaccine

Abstract

Rift Valley Fever is a viral disease that primarily infects animals but it can also infect humans. The disease causes severe clinical manifestations in both animals and humans that might eventually cause death. It also represents an economical burden owing to the abortion storms accompanied by the infection and exporting restrictions at the times of outbreak. For the previously mentioned reasons it’s a necessity to develop safe and efficacious vaccines as vaccination is the primary method to protect the livestock to prevent the outbreaks and the subsequent losses. Conducted experiments • ZH501 Virus propagation on Vero cell lines followed by sterility testing to ensure absence of adventitious microorganism. Followed by virus titration in both BALB/c mice and cell line. • Conducting PCR as a preliminary quality control step to indicate viral identity.