BIOCHEMICAL AND TOXICOLOGICAL STUDIES OF SOME PESTICIDES ON COTTON LEAFWORM SPODOPTERA LITTORALIS
MERY MAGDY SOBHY SHENOUDA;
Abstract
The present study is to through light on role of some insecticides (chemical insecticides (a.i.chlorphyrifos) Dursban® 48% EC, spinosyns group (a.i.spinosad) Tracer® 24% SC and insect growth regulator (a.i.lufenuron) Match® 5% EC) against insect attacking cotton cultivation under Egyptian conditions as follows:
I-Effect of treatment with chlorphyrifos ,spinosad and lufenuron insecticides on 2ndlarval instar of S.littoralis:
The mortality percentage was recorded to calculate the LC50 and LC90 values.
1- The obtaind results showed that the LC25 values were 2.21, 8.1 and 0.0005 ppm for the insecticides chlorphyrifos, spinosad and lufenuron, respectively.
2- Also the LC50 values were 6.25, 29.86 and 0.015 ppm for chlorphyrifos, spinosad and lufenuron, respectively.
3- Moreover the LC90 values were 44.96, 355.98 and 9.38 ppm for the compounds chlorphyrifos, spinosad and lufenuron, respectively.
II- The biochemical effects for tested insecticides:
The biochemical parameters for tested insecticides at LC25were studied on some bio-components namely acetylcholinesterase: (AchE), glutathione s-Transferase: (GST), chitinase, phenoloxidase, aspartate (AST) and alanine (ALT) aminotransferases, α- Esterase (α- Est), total protein and protein electrophoresis for laboratory strain.
• The results can be summarized as follows:
1-Effect of insecticides on acetylcholinesterase (AchE):
The enzyme activity of acetylcholinesterase in insect treated with chlorphyrifos and lufenueon recorded increase in enzyme activity by change value 50% and 200% in comparion with control, respectively.
I-Effect of treatment with chlorphyrifos ,spinosad and lufenuron insecticides on 2ndlarval instar of S.littoralis:
The mortality percentage was recorded to calculate the LC50 and LC90 values.
1- The obtaind results showed that the LC25 values were 2.21, 8.1 and 0.0005 ppm for the insecticides chlorphyrifos, spinosad and lufenuron, respectively.
2- Also the LC50 values were 6.25, 29.86 and 0.015 ppm for chlorphyrifos, spinosad and lufenuron, respectively.
3- Moreover the LC90 values were 44.96, 355.98 and 9.38 ppm for the compounds chlorphyrifos, spinosad and lufenuron, respectively.
II- The biochemical effects for tested insecticides:
The biochemical parameters for tested insecticides at LC25were studied on some bio-components namely acetylcholinesterase: (AchE), glutathione s-Transferase: (GST), chitinase, phenoloxidase, aspartate (AST) and alanine (ALT) aminotransferases, α- Esterase (α- Est), total protein and protein electrophoresis for laboratory strain.
• The results can be summarized as follows:
1-Effect of insecticides on acetylcholinesterase (AchE):
The enzyme activity of acetylcholinesterase in insect treated with chlorphyrifos and lufenueon recorded increase in enzyme activity by change value 50% and 200% in comparion with control, respectively.
Other data
| Title | BIOCHEMICAL AND TOXICOLOGICAL STUDIES OF SOME PESTICIDES ON COTTON LEAFWORM SPODOPTERA LITTORALIS | Other Titles | دراسات بيوكيميائية وسمية لبعض المبيدات على دودة ورق القطن | Authors | MERY MAGDY SOBHY SHENOUDA | Issue Date | 2020 |
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